PEGylated Ribonuclease A on Jupiter® 300 C18 3µm, reaction at different time-points

Application Detail (App ID: 17515)

Part No:00F-4263-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA, 2% ACN in Water
Elution B:90% ACN, 0.085% TFA in Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 25 35 65

Flow Rate:1 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (25°C)
Note:Separation of N-Terminus PEGylated Rebonuclease A with 20kDa Methoxy PEG aldehyde (PEG1) on Jupiter 300 3µm C18.

Analyte Details

Analyte Detail not available

PEGylated Ribonuclease A