A Quick Look at (SEC)
Size Exclusion Chromatography (SEC) is a chromatographic method which separates analytes solely based on their size, where molecules are separated on the basis of their exclusion from pores in the column packing material. Larger analytes will elute first, while the smaller molecules interact more with the stationary phase and will elute later.
Based on the analytes and the mobile phases used, size exclusion chromatography can be broken into two main categories, aqueous size exclusion/gel filtration chromatography (GFC) and non-aqueous size exclusion/gel permeation chromatography (GPC).
Aqueous Size Exclusion/ Gel Filtration Chromatography (GFC) Analyze and/or characterize proteins, peptides, and other biomolecules such as antibodies, immunoglobulins, protein complexes, protein aggregates, and desalting.
Non-Aqueous Size Exclusion/ Gel Permeation Chromatography (GPC)
Analyze polymers such as adhesives, oils, plasticizers, plastics, resins, and rubbers/elastomers.