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Standards and IS, Clarity OTX Central Nervous System (CNS) Tissue

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Sample Preparation Details

Application Detail (App ID: 26262)

Column: bioZen™ 2.6 µm Oligo,LC Column 100 x 2.1 mm, Ea

Part No:00D-4790-AN

Dimensions:100 x 2.1 mm ID

Elution Type:Gradient

Elution A:1.0 % HFIP, 0.1% DIPEA in Water with 10 µm EDTA

Elution B:0.075% HFIP, 0.0375% DIPEA in Water:ACN (35:65) with 10 µm EDTA

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	95	5
10	6.2	80	20
11	6.3	20	80
12	7.5	5	95
13	7.8	5	95
14	7.9	95	5
2	0.1	95	5
3	2.5	80	20
4	4	40	60
5	4.1	5	95
6	4.4	5	95
7	5	80	20
8	5.8	5	95
9	6.1	5	95

Flow Rate:0.5 mL/min

Col. Temp: 80 °C

Detection: LC/MS/MS @ (0°C)

Analyte Details

Analyte Detail not available

Step No.	Time (min)	Pct A	Pct B
1	0	95	5
10	6.2	80	20
11	6.3	20	80
12	7.5	5	95
13	7.8	5	95
14	7.9	95	5
2	0.1	95	5
3	2.5	80	20
4	4	40	60
5	4.1	5	95
6	4.4	5	95
7	5	80	20
8	5.8	5	95
9	6.1	5	95

Step No.	Time (min)	Pct A	Pct B
1	0	95	5
10	6.2	80	20
11	6.3	20	80
12	7.5	5	95
13	7.8	5	95
14	7.9	95	5
2	0.1	95	5
3	2.5	80	20
4	4	40	60
5	4.1	5	95
6	4.4	5	95
7	5	80	20
8	5.8	5	95
9	6.1	5	95

Step No.	Time (min)	Pct A	Pct B
1	0	95	5
10	6.2	80	20
11	6.3	20	80
12	7.5	5	95
13	7.8	5	95
14	7.9	95	5
2	0.1	95	5
3	2.5	80	20
4	4	40	60
5	4.1	5	95
6	4.4	5	95
7	5	80	20
8	5.8	5	95
9	6.1	5	95