Purification Products

Clarity (SPE) Sample Preparation Information

Isolation and Purification of Synthetic Oligonucleotides

The Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to efficiently and effectively purify oligonucleotides.

  • Works for desalting and clean-up of biological samples
  • Specially formulated buffers solutions available for optimal performance
  • Quick protocols for rapid results

Clarity BioSolutions Product Selection


  Clarity QSP™ Clarity Oligo-RP™
Clarity Oligo-XT
Clarity Oligo-SAX™ Clarity RP-Desalting™
Primary Use High-throughput, trityl-on RPC
RP-HPLC purification of failure
sequences from target sequences
Economical, high loading capacity
IEX-LC prep-scale purification
Quick removal or salt & excess reagent
Purities >90% >90% >90% ~70%
Recoveries ~90% ~70% >90% ~70%
Synthesis Scale Load Up to 50 µmol Up to 50 µmol Up to 50 µmol Up to 1 µmol
Oligo Types DNA, RNA/RNAi, Thioates, Dye-labled, Modified

Characterization / Analysis

  Clarity Oligo-RP™ Clarity Oligo-MS™
Clarity Oligo-XT
Clarity Oligo-OTX™
Primary Use RP-LC-MS analysis with optimized
selectivity and sensitivity
Rapid, high efficiency RP-LC-MS
analysis for QC and characterization
Extraction of oligo therapeutics from
biological samples for LC-MS bioanalysis
Oligo Length ≤ 60 mer ≤ 60 mer ≤ 40 mer
Recommended Mobile Phase TEA / HFIP TEA/HFIP/MeOH N/A

Material Characteristics

Clarity Products Particle
Pore Size
Clarity QSP™ Cartridges Polymer (PSDVB) Hydrophilic polymer coating 35 500 300
Clarity Oligo-RP™
LC Columns
(silica, organic, composite)
C18 3, 5, 10 110 375
Clarity Oligo-WAX™
LC Columns
Silica Crosslinked polyamine (WAX) 10 360
Clarity RP-Desalting™ Tubes Silica C18 55 140 300
Clarity Oligo-MS™
LC Columns
Core-Shell C18 1.7, 2.6, 5 100 200*
Clarity OTX™
Extraction Plates
Polymer (surface
modified PSDVB)
Mixed-mode anion exchanger 33 85 800
Clarity Oligo-SAX
LC Columns
Polymer (surface
modified PSDVB)
Hydrophilic quaternary amine 5
Clarity Oligo-XT
LC Columns
Core-Shell C18 1.7, 2.6, 5 100 200

QSP Phase Detail

QSP Phase Information

High-Throughput Purification

Purify trityl-on synthetic DNA or RNA samples in 20 minutes using Clarity QSP™ 96-well plate and buffer system. Final elution delivers >90 % typical purities/recoveries for in vivo applications and downstream analysis by LC/MS or CE.

  • Formats offer easy high throughput processing for parallel purifications
  • Simple three-step process delivers highly purified DNA/RNA in minutes
  • For oligos 10-100 mer
R&D 100 Logo
2008 R&D 100 Award Recipient

Suitable for Most Oligo Therapeutics & Samples

Formats: 96-Well Plates and 3 mL Cartridges
Oligo Chemistry: DNA & RNA
Synthesis Scale Load:
  • 50 mg/well 96-well plates and 60mg/3mL tubes=up to 200 nmole
  • 150 mg/ 3ml tubes= up to 1 µmole
  • 5g/60mL tubes= up to 25 µmole
Oligo Length: ≤ 100 nt
Typical Purity1: ≥ 90 %
Typical Recovery Yield2: ≥ 80 %
Purification Time: ~ 8 minutes/ cartridge
~ 45 minutes/ well plate (96 samples)
Equipment Required: Positive pressure manifold, vacuum manifold, or automated liquid handling system

1 Purity value based on ion exchange chromatography and capillary electrophoresis

2OD260 used for quantitation

Phase: Sorbent Mass:

Part Number:8B-S042-LFF

Description:Clarity® 70 µm QSP, 5 g / 60 mL, Giga Tubes , 16/Pk

Sorbent Type:Polymer-based



Target Analytes:Oligos 10 – 100 mer

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