Region

Oligonucleotide

Purification Products

Clarity (SPE) Sample Preparation Information


Isolation and Purification of Synthetic Oligonucleotides

The Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to efficiently and effectively purify oligonucleotides.

  • Works for desalting and clean-up of biological samples
  • Specially formulated buffers solutions available for optimal performance
  • Quick protocols for rapid results

Clarity BioSolutions Product Selection

Purification

  Clarity QSP™ Clarity Oligo-RP™
Clarity Oligo-XT
Clarity Oligo-SAX™ Clarity RP-Desalting™
Primary Use High-throughput, trityl-on RPC
purification
RP-HPLC purification of failure
sequences from target sequences
Economical, high loading capacity
IEX-LC prep-scale purification
Quick removal or salt & excess reagent
Purities >90% >90% >90% ~70%
Recoveries ~90% ~70% >90% ~70%
Synthesis Scale Load Up to 50 µmol Up to 50 µmol Up to 50 µmol Up to 1 µmol
Oligo Types DNA, RNA/RNAi, Thioates, Dye-labled, Modified

Characterization / Analysis

  Clarity Oligo-RP™ Clarity Oligo-MS™
Clarity Oligo-XT
Clarity Oligo-OTX™
Primary Use RP-LC-MS analysis with optimized
selectivity and sensitivity
Rapid, high efficiency RP-LC-MS
analysis for QC and characterization
Extraction of oligo therapeutics from
biological samples for LC-MS bioanalysis
Oligo Length ≤ 60 mer ≤ 60 mer ≤ 40 mer
Recommended Mobile Phase TEA / HFIP TEA/HFIP/MeOH N/A

Material Characteristics

Clarity Products Particle
Support
Bonded
Phase
Particle
shape/Size
(µm)
Pore Size
(Å)
Surface
Area
(m2/g)
Clarity QSP™ Cartridges Polymer (PSDVB) Hydrophilic polymer coating 35 500 300
Clarity Oligo-RP™
LC Columns
TWIN
(silica, organic, composite)
C18 3, 5, 10 110 375
Clarity Oligo-WAX™
LC Columns
Silica Crosslinked polyamine (WAX) 10 360
Clarity RP-Desalting™ Tubes Silica C18 55 140 300
Clarity Oligo-MS™
LC Columns
Core-Shell C18 1.7, 2.6, 5 100 200*
(*effective)
Clarity OTX™
Extraction Plates
Polymer (surface
modified PSDVB)
Mixed-mode anion exchanger 33 85 800
Clarity Oligo-SAX
LC Columns
Polymer (surface
modified PSDVB)
Hydrophilic quaternary amine 5
Clarity Oligo-XT
LC Columns
Core-Shell C18 1.7, 2.6, 5 100 200

QSP Phase Detail


QSP Phase Information


High-Throughput Purification

Purify trityl-on synthetic DNA or RNA samples in 20 minutes using Clarity QSP™ 96-well plate and buffer system. Final elution delivers >90 % typical purities/recoveries for in vivo applications and downstream analysis by LC/MS or CE.

  • Formats offer easy high throughput processing for parallel purifications
  • Simple three-step process delivers highly purified DNA/RNA in minutes
  • For oligos 10-100 mer
R&D 100 Logo
2008 R&D 100 Award Recipient

Suitable for Most Oligo Therapeutics & Samples

Formats: 96-Well Plates and 3 mL Cartridges
Oligo Chemistry: DNA & RNA
Synthesis Scale Load:
  • 50 mg/well 96-well plates and 60mg/3mL tubes=up to 200 nmole
  • 150 mg/ 3ml tubes= up to 1 µmole
  • 5g/60mL tubes= up to 25 µmole
Oligo Length: ≤ 100 nt
Typical Purity1: ≥ 90 %
Typical Recovery Yield2: ≥ 80 %
Purification Time: ~ 8 minutes/ cartridge
~ 45 minutes/ well plate (96 samples)
Equipment Required: Positive pressure manifold, vacuum manifold, or automated liquid handling system

1 Purity value based on ion exchange chromatography and capillary electrophoresis

2OD260 used for quantitation

Document Type:
Document Type Title
Technical Notes Automated Method Development of Oligonucleotide in Tissues Using Clarity OTX 96-Well Plate and High Resolution Mass Spectrometry (TN-0097) Add to My Documents
Brochures Clarity Biosolutions for Synthetic DNA/RNA Add to My Documents
Brochures Clarity QSP Add to My Documents
MSDS AL0-8280_Clarity QSP DNA Buffer SDS Add to My Documents
MSDS AL0-8282_Clarity QSP RNA Buffer SDS Add to My Documents
MSDS AL0-8579_Clarity OTX Lysis Loading Buffer SDS Add to My Documents
Technical Notes Avoiding Depurination During Trityl-on Purification (TN-0008) Add to My Documents
Brochures Clarity OTX Add to My Documents
Supporting Docs Clarity OTX User Guide Add to My Documents
Supporting Docs Clarity QSP User’s Guide (DNA) Add to My Documents
Supporting Docs Clarity QSP User’s Guide (RNA) Add to My Documents
Technical Notes Trityl-on RNA and DNA Purification (TN-0009) Add to My Documents
MSDS KS0-8494_Clarity OTX Elution Buffer SDS (for kit) Add to My Documents
MSDS KS0-8494_Clarity OTX Equilibration Buffer SDS (for kit) Add to My Documents
MSDS KS0-8494_Clarity OTX Wash Buffer SDS Add to My Documents
MSDS KS0-8494_Clarity OTX Wash Buffer SDS (for kit) Add to My Documents
Technical Notes Oligonucleotide Therapeutics from Biological Matrices (TN-0048) Add to My Documents
Supporting Docs Clarity Overview Flyer Add to My Documents
Supporting Docs Collection Plate and Sealing Mat Selection Guide Add to My Documents
Other Protein and Peptide Users Guide Add to My Documents
Technical Notes RNA Sample Preparation and High Throughput Purification for TBDMS and TOM Chemistries (TN-0016) Add to My Documents
Supporting Docs SPE 10, 12, and 24 Position Vacuum Manifold Instructions Add to My Documents
Supporting Docs SPE 96-Well Vacuum Manifold Add to My Documents
Phase: Sorbent Mass:
Loading...

Part Number:8B-S042-LFF

Description:Clarity® 70 µm QSP, 5 g / 60 mL, Giga Tubes , 16/Pk

Sorbent Type:Polymer-based

Format:Tube

Phase:DVB

Target Analytes:Oligos 10 – 100 mer


 Search Results


Part
Number
Brand Phase Sorbent Mass
/Volume
Format Price