Jupiter® 300 C4 - Effect of Temperature on PEGylated Protein Separations

Application Detail (App ID: 16196)

Part No:00F-4167-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:90% ACN 0.09% TFA in Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 25 45 55

Flow Rate:1 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:Reversed phase runs of PEGylated carbonic anhydrase at different temperatures. Increasing the temperature to 45°C slightly improves resolution of different PEG forms.

Analyte Details

Analyte Detail not available

PEGylated Carbonic anhydrase